P 202 Title: Efficient and noninvasive in vivo gene transfer technique into adult mammalian ocular tissue

Purpose To investigate the peripheral blood T-cell subpopula-tions in patients with different forms of uveitis. Met/rods The CD 3+, CD 4+ and CD 8+ lymphocyte subsets were studied in peripheral blood by flowcflomeby in 220 patients with anterior, intermediate, posterior and panuveitis (150 cases with proven etiology and underlined clinical syndrome and 70 with idiopathic uveitis). The control group consisted of 30 healthy subjects.. Results The CD 4ICD 8 index was increased in 50% of patients with anterior uveitis, associated with systemic diseases, as it was seen in intermediate uveitis, while the idiopathic forms of the two groups did not exhibit any changes. The CD 8+ cells in active stage of toxoplasmic retinochoroiditis and the CD 4+ cells in TBC uveitis were significantly increased, compared to healthy controls. In almost all cases with Behcet's disease we found a decreased number of panT lymphocytes and disturbances mostly in CD 8+ cells. Conclusions: A T-cell immune di:sbalance in peripheral blood was found predominantly in patients with uveitis, associated with diseases, accompanied by systemic immune manifestations P200 IMMUNOHISTOCHEMICAL DISTRIBUTION OF FK506 BINDING PROTEIN IN HUMAN OCIJLAR TISSUES. eymnSa: FK506 binding protein-l:! (FKBP-12). the 12.KDa. intracellular receptor for the immunosup:?ressive drug FK506 is widely dishibuted in eukaryote cells, but its localization in ocular tissues has not been investigated. Therefore, the distribution of FKBP-12 in ocular tissues was examined using immunohistochemical technique. w Human eye ball was obtained fmm the autopsy in Juntendo University Hospital. Two kinds of monoclonal antibodies (2Cl and 3F4) which have different epitopes of FKBP-12 were given by Fujisawa Pharmaceutical Co. Ltd.. The eye ball was divided into several pieces and were snap-frozen wil h liquid nitrogen. The frozen tissues were cut into 5pm sections and fixed in acetone. Then, sections were incubated with the antibodies 2C1 or 3F4 to FKBP-12. Immunohistochemical study was done uing a horseradish peroxidase method. m: Positive reactions were swongly detected with both antibodies in cornea1 epithelium. But the positive staining of the antibody 2Cl seemed to localize in the m~leus and that of the antibody 3F4 was observed to localize in the cytoplasm of the comeal epithelium. Weak reactions were alsodetected with tile antibody 2Cl in the nucleus of cornea1 keratocytes. On the other hand, the cytoplasm of the non-pigmented epithelium of the ciliary body was strongly stained with both antibodies. No reaction was observed to anti-FKBP-12 antibodies in iris and trabecular meshwork. m: It …